Evaluation of Crude Extract of Argemone mexicana and Buddleja polystachya on Wound Healing

Abstract

This experimental study was conducted from November 2018 to May 2019 with the objective of evaluating the in vivo wound healing activity of Argemone mexicana seed and Buddleja polystachya seed extracts. A total of 16 white albino mice were grouped into four groups (G-I= received no any treatment; G-II= treated with simple ointment; GIII=treated with 10% B. polystachya seed formulated extract; and G-IV= treated with 10% A. mexicana formulated seed extract) each containing four mice. An excisional wound was created on the dorsal area/back of the mice. Plant extracts were prepared and evaluated for wound healing activity. The wound 
healing activity of each plant extract was evaluated based on wound contraction/gap filled and histopathological changes. All the data were analyzed using STATA version-13.0 statistical software. The mean ± SD of wound contraction was evaluated to determine the wound healing activity in each treatment. By day 12, treatment with 10% ointment of B. polystachya seed extract and A. mexicana enabled the wound to heal at faster rate with mean±SD of 0.20±0.00 and 0.00±0.00, respectively. Wound healing was significantly higher in groups treated with B. polystachya (p=0.001) and A. mexicana (p=0.00) compared to the control group (G-I). 
Histological examination of the wounds treated with A. mexicana and B. polystachya extract ointment revealed reduced period of inflammation and enhanced fibroblast proliferation, angiogenesis and epithelialization compared to the control group. Both 10% ointment ethanolic extract of the plant seeds showed significant responses. Thus, ethanolic extract of A. mexicana and B. polystachya proved to have a potential in wound healing activity.